The 16 Premier League clubs will join the 48 Division One and Two teams that qualified from the preliminary round to determine the pairings for the Round of 64.

Defending champions, Bechem United, will join the draw as they begin their title defence.

Teams that have qualified for the draw are as follows: 

Ashanti: Kumasi Asante Kotoko, Asokwa Deportivo, King Faisal, Achiken FC, Atwima Mahoni, Bekwai Youth Football Academy, Ashantigold, Bibiani Gold Stars

Northern/Upper East: Bolga Soccer Stars, Guan United, Real Tamale United, Tamale Utrecht Academy, Steadfast FC, Bolga All Stars

Brong Ahafo: Techiman Eleven Wonders, Nea Salamina, Unity FC, Nsoatre FC, Glory Stars, B.A United, Aduana, Chelsea and Bechem United.

Upper West: Power Sporting Club, Wa All Stars Dark Horses, Kintampo FC.

Central: Unistar Academy, Proud Utd, Fetteh Youngsters, Elmina Sharks, Ebusua Dwarfs, Windy Professionals

Volta: Hearts of Lions, Nania FC, ALKE FC, WAFA, Uncle T Stars, NADM FC

Western: Dolphins FC, Eleven Wise/New Edubiase, Nzema Kotoko, Wassaman, FC Samartex, Rail Stars, Fijai Real Utd, Medeama

Eastern: Kotoko Royals, Okyeman Planners, Phar Rangers, Dreams FC.