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James Maddison, Jadon Sancho and Mason Mount have been named in an England squad for the first time.
Leicester City playmaker Maddison, Borussia Dortmund winger Sancho and Derby's on-loan midfielder Mount are in the squad for the Nations League games against Croatia and Spain in October.
Sancho is the first player born this century to be called up by England.
Chelsea midfielder Ross Barkley returns to the squad for the first time since May 2016.
There are six uncapped players in total in boss Gareth Southgate's 25-man squad, which has an average age of 23.9.
Fulham keeper Marcus Bettinelli, Southampton counterpart Alex McCarthy and Watford midfielder Nathaniel Chalobah are the other uncapped players.
Tottenham midfielder Harry Winks, whose only cap came against Lithuania in October 2017, returns to the squad.
"It aligns with what we have done in the last 18 months," said Southgate.
"We've got a few injuries in the midfield in particular - Dele [Alli], Jesse [Lingard] and Ruben [Loftus-Cheek] have been missing - and Fabian Delph, so there is an opportunity there.
"Then we have a decision on which way we want to use that opportunity. We think there are some exciting young players that we've been tracking, either through their clubs or our junior teams.
"This is a great opportunity for us to look at them."
Maddison, 21, Sancho, 18, and Mount, 19, have been in impressive form so far this season
Maddison, 21, who has won four England Under-21 caps, was watched by Southgate when he helped the Foxes beat Newcastle United on Saturday.
He has three goals and two assists for Leicester since a £20m summer move from Norwich.
Sancho, 18, has assisted in five league goals for Dortmund this season as he has helped them top the Bundesliga after six games.
He joined the German side from Manchester City for about £10m in August 2017 and signed a new deal earlier this week.
Mount has been impressive for Championship side Derby, where he is on loan from Chelsea, with the 19-year-old having scored five goals this season.
"Jadon's is an unusual story for a young English player. He's been brave enough to go abroad and play," added Southgate.
"He is a player that excites us a lot, he is adapting to a big football club and he has had two starts in the Champions League this year.
"He's a player that is explosive, is really creative in the final third, and has had a big impact in terms of assists in the matches he has played, can score goals and has had a big impact in our junior teams in the last couple of years.
"Mason's is a slightly different situation. He had a year at [Dutch side] Vitesse Arnhem last year so he's had another year of men's football. This year in the Championship he's created more chances than any other player in the league.
"They're both still at formative stages but we think they can have an impact with the group and we'd have no hesitation in playing them in the games."
Goalkeepers
Marcus Bettinelli (Fulham), Jack Butland (Stoke City), Alex McCarthy (Southampton), Jordan Pickford (Everton)
Defenders
Trent Alexander-Arnold (Liverpool), Joe Gomez (Liverpool), Harry Maguire (Leicester City), Danny Rose (Tottenham Hotspur), Luke Shaw (Manchester United), John Stones (Manchester City), James Tarkowski (Burnley), Kieran Trippier (Tottenham Hotspur), Kyle Walker (Manchester City)
Midfielders
Ross Barkley (Chelsea), Nathaniel Chalobah (Watford), Eric Dier (Tottenham Hotspur), Jordan Henderson (Liverpool), Mason Mount (on loan at Derby County from Chelsea), Harry Winks (Tottenham Hotspur)
Forwards
Harry Kane (Tottenham Hotspur), James Maddison (Leicester City), Marcus Rashford, (Manchester United), Jadon Sancho (Borussia Dortmund), Raheem Sterling (Manchester City), Danny Welbeck (Arsenal)
