Defender John Stones has withdrawn from the England squad for the next two World Cup qualifiers because of injury.
Manager Thomas Tuchel says he does not want to take any risks with the 31-year-old, who arrived at the training camp with a "muscular issue" that has not improved.
Stones has started all three of Manchester City's Premier League games this season.
Tuchel said: "He came with minor issues and didn't progress as we thought and hoped he would, so he left camp this morning.
"Everyone else is available. We had 21 players yesterday in training and 21 players today in training and hopefully make sure everyone is available for tomorrow."
Marc Guehi, Dan Burn, Ezri Konsa and Jarell Quansah are Tuchel's other centre-back options.
England play Andorra at Villa Park on Saturday before travelling to Serbia on Tuesday.
Stones being ruled out for these World Cup qualifying matches is a blow for Tuchel.
Tuchel is a huge admirer of the talent that Stones has and has spoken about the unique qualities that he can bring to any England side.
Stones has yet to play for Tuchel since he became England manager and missed the last two camps through injury.
And injury has again robbed Tuchel of Stones' talent and his experience will be missed when England head to Serbia next week for their toughest match of the World Cup qualifying campaign.
Tuchel has been trying to get his tactical message across to the players this week and is still trialing his midfield options in his third camp, and Stones was one of the players he thinks could fit there.
The manager referenced that Stones could be a number six option as England are without a natural player of that type in the squad.
The German is now going to have to hope that the muscular issues that have forced Stones to pull out are not too serious and that he can include the City defender in his squad in October.